Journal: Microorganisms
Article Title: Development of a Strain-Specific Detection and Quantification Method for Bifidobacterium animalis subsp. lactis HN019 Using WGS-SNP Analysis and qPCR
doi: 10.3390/microorganisms13071596
Figure Lengend Snippet: Figure 4. Repeatability (a) and reproducibility (b) test for specific detection method of B. animalis subsp. lactis HN019 strain. Violin plots were generated using GraphPad Prism 9, Median (dashed line), kernel density distribution (blue line or purple line) are shown. (a) Repeatability test, show- ing three trials with three replicates (dark blue dots) in each trial, for a total of 9 measurements. (b) Reproducibility test, showing experiments conducted on two different qPCR platforms (Roche LightCycler® 480 and ABI 7500), for a total of 6 measurements (dark purple dots).
Article Snippet: For reproducibility testing across two different qPCR platforms (Roche LightCycler® 480 and ABI 7500), the RSD ranged from 1.55% to 2.65% (Figure 4b), which is well below the requirement that qPCR detection results should have an RSD value of less than 25% [36].
Techniques: Generated